maturing gene meaning in English

成熟基因

The mature gene of gloshedobin was inserted into the expression vector ppic9k . the yeast pichia pastoris strain gs115 was applied to study the expression
经sds - page ，亲和层析及电镜分析，表达产摘蛰物主要以可溶性的蛋白质形式存在于细胞质中。
Both the mature genes of gloshedobin and gussurobin were cloned into the vector pet - 32a ( + ) , strain bl21 ( de3 ) , to study their expression in prokaryotic cell . the gene was expressed under t7 promoter with a fusion protein partner of thx . tag and a 6x his . tag at its n - terminal . having been induced by iptg for 4 hours , the recombinant enzyme was examined in the cytoplasm by sds - page analysis
将大连蛇岛蝮蛇和长白山白眉蝮蛇毒类凝血酶基因分别克隆到大肠杆菌表达载体pet - 32 ( a ) +中，在t7启动子下表达出融合蛋白，融合部分为硫氧还蛋白，位于类凝血酶基因上游，并在其n端带6xhistag标签以利于表达产物的分离纯化，经热激转化至宿主菌bl21 ( de3 )中， iptg诱导斗小时后收获菌体。